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KMID : 0900919970210010001
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Korean journal of Animal Reproduction
1997 Volume.21 No. 1 p.1 ~ p.7
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A Study on In Vitro Model for Mammary-Specific Gene Expression
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Abstract
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Recently the production of transgenic animals to express foreign proteins in mammary glands has been a routine procedure. However, it still takes a considerable time and effort, and is faced with various technical challenges until the protein of interest is successfully made. Thus, a development of an a vitro model for mamm a ary-specific gene expression for recombinant genes was carried out in this study. To this end, bovine alpha_S1 casein cDNA was inserted at the multiple cloning site of pMSG vector under the control of MMTV promoter. MCF_7 cells were tran sfected with pMSG alpha_S1 CN by CaP0_4 precipitation. Transfectants were selected in HAT medium and induced with dexamethasone. The cells were analyzed with chicken anti-casein and FITC-labeled rabbit anti-chicken antibodies. The results showed that dexamethasone induced 30-40 fold increase in the MMTV- alpha_S1 casein e expression. Therefore MCF_7 cells, which have multiple steroid receptors, along with pMSG vector can be used as an in vitro model for the study of mammary-specific gene expression.
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KEYWORD
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In vitro model, Mammary-specific gene, Transgenic animal, MCF_7 cell line
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